Hello : ) This is Lakshmi from India. I have a doubt while doing isolation of DNA from a bacterial cell.We were given a protocol and it says that we have to resusupend bacterial pellet in SET buffer, then add SDS , then add NaCl, and then add NaI and 20% Isopropanol.Spin down the cells.To the supernatant add Silica and stay for a while and centrifuge it .Finally wash the pellet with 70% ethanol and resuspend the pellet in TE buffer.
 
I dont know how the RNA and Plasmid DNA is going away ?Can you please help me how to figure out this?
 
Thanks a lot :)
 
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